Defining Cell Culture Conditions to Drive Cell Identity and Scalability in Cell Therapy
Pluripotent stem cells (PSCs) represent a valuable resource for the generation of cell types for treating these diseases. These include cardiomyocytes, pancreatic beta cells, and hepatocytes, which have therapeutic potential to treat heart disease, diabetes, and liver failure. Many studies have shown that PSC-derived cardiomyocytes, pancreatic beta cells, and hepatocytes can be transplanted in animal models, and are capable of engrafting and providing a functional benefit. However, the development of cell therapies is tightly linked to our ability to culture cells in artificial environments outside of the body, i.e., in vitro conditions. In our bodies, cells live in highly regulated and specialized microenvironments, also known as niches. These niches provide adequate conditions for cells to perform their function, maintain their cellular identity and, whenever required, divide and respond to their surroundings.
This article explores using epigenetically predicted factors to mimic in vivo microenvironments and systematically define in vitro culture conditions for optimal cell maintenance and differentiation.View all resources